Journal: Oncology reports

Article Title: Lin28/let-7/Bcl-xL pathway: the underlying mechanism of drug resistance in Hep3B cells.

doi: 10.3892/or.2014.3292

Figure Lengend Snippet: Figure 5. The expression of Lin28 and let-7 family in Hep3B and Hep3B/TAX cells. (A) Lin28 mRNA was examined by qPCR in Hep3B and Hep3B/TAX cells. (B) Lin28 expression was determined in Hep3B and Hep3B/TAX cells separately by western blotting; β-actin expression was used as a control. (C) Expression of let-7 family miRNAs by qPCR. **P<0.001 Hep3B/TAX compared with Hep3B cells.

Article Snippet: Antibodies against Lin28 and β-actin, and horseradish peroxidase-conjugated secondary antibodies were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Molecular cell

Article Title: A Rapid Induction Mechanism for Lin28a in Trophic Responses

doi: 10.1016/j.molcel.2016.12.025

Figure Lengend Snippet: (A) Quantification (top) and representative immunoblot (bottom) of Lin28a protein levels in lysates from hippocampal neurons exposed to BDNF with or without transcription blockade (Actinomycin-D) for the indicated times, normalized to GAPDH and plotted relative to 0-min BDNF without Actino-mycin-D (set as 1.0). #p < 0.05, ANOVA; *p < 0.05, t test.

Article Snippet: Rabbit polyclonal anti-Lin28a , LifeSpan Biosciences , Cat#LS-C165782 now LS- {"type":"entrez-nucleotide","attrs":{"text":"B11566","term_id":"2092687","term_text":"B11566"}} B11566.

Techniques: Western Blot

Journal: Molecular cell

Article Title: A Rapid Induction Mechanism for Lin28a in Trophic Responses

doi: 10.1016/j.molcel.2016.12.025

Figure Lengend Snippet: (A) Representative immunoblot (left) and quantified Lin28a protein levels (right) normalized to HSC70 from immunoblot of lysates from hippocampal neurons pre-treated with vehicle (DMSO) or the MEK/Erk inhibitor U0126 (30 min) prior to BDNF. Erk1/2 inhibition prevents Lin28a induction by BDNF (#p < 0.01, ANOVA) and decreases basal Lin28a protein levels (*p < 0.05, t test).

Article Snippet: Rabbit polyclonal anti-Lin28a , LifeSpan Biosciences , Cat#LS-C165782 now LS- {"type":"entrez-nucleotide","attrs":{"text":"B11566","term_id":"2092687","term_text":"B11566"}} B11566.

Techniques: Western Blot, Inhibition

Journal: Molecular cell

Article Title: A Rapid Induction Mechanism for Lin28a in Trophic Responses

doi: 10.1016/j.molcel.2016.12.025

Figure Lengend Snippet: (A) Representative immunoblot (left) and densitometric quantification (right) of purified Lin28a protein co-associated with purified GST-TRBP, normalized to TRBP pull-down and plotted relative to GST-TRBPWT (set as 1.0). A lower band in the GST-TRBP conditions that migrates similarly to GST alone reflects partial cleavage of the GST tag through a protease site between GST and TRBP. Uncropped blots are shown in Figure S4A.

Article Snippet: Rabbit polyclonal anti-Lin28a , LifeSpan Biosciences , Cat#LS-C165782 now LS- {"type":"entrez-nucleotide","attrs":{"text":"B11566","term_id":"2092687","term_text":"B11566"}} B11566.

Techniques: Western Blot, Purification

Journal: Molecular cell

Article Title: A Rapid Induction Mechanism for Lin28a in Trophic Responses

doi: 10.1016/j.molcel.2016.12.025

Figure Lengend Snippet: Shown is a graphical model. Left: at baseline, TRBP undergoes polyubiquitination and degradation facilitated by Merlin protein. Lin28a protein levels are negligible as it undergoes rapid turnover. Right: growth factor binding to cognate receptors activates Erk1/2 signaling and subsequent TRBP phosphorylation, which stabilizes TRBP and enhances the TRBP/Lin28a protein complex, elevating Lin28a protein levels. Downstream inhibition of Let-7 miRNAs facilitates protein synthesis from pro-growth mRNAs. Bottom: The rapid Erk1/2-dependent induction pathway exerts Lin28a-dependent trophic effects on neuronal spines and peritoneal macrophage survival.

Article Snippet: Rabbit polyclonal anti-Lin28a , LifeSpan Biosciences , Cat#LS-C165782 now LS- {"type":"entrez-nucleotide","attrs":{"text":"B11566","term_id":"2092687","term_text":"B11566"}} B11566.

Techniques: Binding Assay, Inhibition

Journal: Molecular cell

Article Title: A Rapid Induction Mechanism for Lin28a in Trophic Responses

doi: 10.1016/j.molcel.2016.12.025

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Lin28a , LifeSpan Biosciences , Cat#LS-C165782 now LS- {"type":"entrez-nucleotide","attrs":{"text":"B11566","term_id":"2092687","term_text":"B11566"}} B11566.

Techniques: Recombinant, Protease Inhibitor, TaqMan microRNA Assay, Plasmid Preparation, shRNA, Software